Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing.

ABSTRACT:

Background

Dysfunction or deficiency of corneal epithelium results in vision impairment or blindness in severe cases. The rapid and effective regeneration of corneal epithelial cells relies on the limbal stem cells (LSCs). However, the molecular and functional responses of LSCs and their niche cells to injury remain elusive.

Methods

Single-cell RNA sequencing was performed on corneal tissues from normal mice and corneal epithelium defect models. Bioinformatics analysis was performed to confirm the distinct characteristics and cell fates of LSCs. Knockdown of Creb5 and OSM treatment experiment were performed to determine their roles of in corneal epithelial wound healing.

Results

Our data defined the molecular signatures of LSCs and reconstructed the pseudotime traject

SUBMITTER: Sun D 

PROVIDER: S-EPMC11227725 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature