Single-cell analysis of human nasal mucosal IgE antibody secreting cells reveals a newly minted phenotype.

ABSTRACT: Immunoglobulin (Ig) E is central to the pathogenesis of allergic conditions, including allergic fungal rhinosinusitis. However, little is known about IgE antibody secreting cells (ASCs). We performed single-cell RNA sequencing from cluster of differentiation (CD)19⁺ and CD19^- ASCs of nasal polyps from patients with allergic fungal rhinosinusitis (n = 3). Nasal polyps were highly enriched in CD19⁺ ASCs. Class-switched IgG and IgA ASCs were dominant (95.8%), whereas IgE ASCs were rare (2%) and found only in the CD19⁺ compartment. Through Ig gene repertoire analysis, IgE ASCs shared clones with IgD^-CD27^- "double-negative" B cells, IgD⁺CD27⁺ unswitched memory B cells, and IgD^-CD27⁺ swi

SUBMITTER: Ramonell RP 

PROVIDER: S-EPMC11227847 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature