Unified metagenomic method for rapid detection of microorganisms in clinical samples.

ABSTRACT:

Background

Clinical metagenomics involves the genomic sequencing of all microorganisms in clinical samples ideally after depletion of human DNA to increase sensitivity and reduce turnaround times. Current human DNA depletion methods preferentially preserve either DNA or RNA containing microbes, but not both simultaneously. Here we describe and present data using a practical and rapid mechanical host-depletion method allowing simultaneous detection of RNA and DNA microorganisms linked with nanopore sequencing.

Methods

The human cells from respiratory samples are lysed mechanically using 1.4 mm zirconium-silicate spheres and the human DNA is depleted using a nonspecific endonuclease. The RNA is converted to dsDNA to allow the simultaneous sequencing of DNA and RNA.

Results<

SUBMITTER: Alcolea-Medina A 

PROVIDER: S-EPMC11228040 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature