A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses.

ABSTRACT: 3D tumoroids have revolutionized in vitro/ex vivo cancer biology by recapitulating the complex diversity of tumors. While tumoroids provide new insights into cancer development and treatment response, several limitations remain. As the tumor microenvironment, especially the immune system, strongly influences tumor development, the absence of immune cells in tumoroids may lead to inappropriate conclusions. Macrophages, key players in tumor progression, are particularly challenging to integrate into the tumoroids. In this study, we established three optimized and standardized methods for co-culturing human macrophages with breast cancer tumoroids: a semi-liquid model and two matrix-embedded models tailored for specific applications. We then tracked interactions and macrophage infiltration in

SUBMITTER: Raffo-Romero A 

PROVIDER: S-EPMC11228374 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature