Copy number and sequence variation in rDNA of Daphnia pulex from natural populations: insights from whole-genome sequencing.

ABSTRACT: Ribosomal DNA (rDNA) has a vital role in ribosome biogenesis as it contains the genes that encode ribosomal RNA (rRNA) separated by intergenic spacers (IGSs). The rRNA genes occur in hundreds to tens of thousands of copies per haploid genome in eukaryotes and are generally highly conserved with low variation within species. Due to the repetitive nature and large size of rDNA arrays, detecting intraindividual variation can be difficult. In this study, we use whole-genome sequences of 169 Daphnia pulex individuals from 10 natural populations to measure the copy number and sequence variation in rDNA. This revealed that variation in rDNA copy number between individuals spans an order of magnitude. We further observed a substantial level of sequence variation within individual genomes. As expec

SUBMITTER: Elguweidi A 

PROVIDER: S-EPMC11228840 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature