TWISP: a transgenic worm for interrogating signal propagation in Caenorhabditis elegans.

ABSTRACT: Genetically encoded optical indicators and actuators of neural activity allow for all-optical investigations of signaling in the nervous system. But commonly used indicators, actuators, and expression strategies are poorly suited for systematic measurements of signal propagation at brain scale and cellular resolution. Large-scale measurements of the brain require indicators and actuators with compatible excitation spectra to avoid optical crosstalk. They must be highly expressed in every neuron but at the same time avoid lethality and permit the animal to reach adulthood. Their expression must also be compatible with additional fluorescent labels to locate and identify neurons, such as those in the NeuroPAL cell identification system. We present TWISP, a transgenic worm for interrogating s

SUBMITTER: Sharma AK 

PROVIDER: S-EPMC11228852 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature