Direct Identification of Intact Proteins Using a Low-Resolution Mass Spectrometer with CIDⁿ/ETnoD.

ABSTRACT: Over the past decades, proteomics has become increasingly important and a heavily discussed topic. The identification of intact proteins remains a major focus in this field. While most intact proteins are analyzed using high-resolution mass spectrometry, identifying them through low-resolution mass spectrometry continues to pose challenges. In our study, we investigated the capability of identifying various intact proteins using collision-induced dissociation (CID) and electron transfer without dissociation (ETnoD). Using myoglobin as our test protein, stable product ions were generated with CID, and the identities of the product ions were identified with ETnoD. ETnoD uses a short activation time (AcT, 5 ms) to create sequential charge-reduced precursor ion (CRI). The charges of the fragme

SUBMITTER: Kuo CY 

PROVIDER: S-EPMC11228978 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature