TRF-His-GTG-1 enhances NETs formation and interferon-α production in lupus by extracellular vesicle.

ABSTRACT:

Background

Hyperactive neutrophil extracellular traps (NETs) formation plays a crucial role in active severe systemic lupus erythematosus (SLE). However, what triggers the imbalance in dysregulated NETs formation in SLE is elusive. Transfer RNA-derived small RNAs (tsRNAs) are novel non-coding RNAs, which participate in various cellular processes. We explore the role of tsRNAs on NETs formation in SLE.

Methods

We analyzed the levels of NETs DNA and platelet-derived extracellular vesicles (pEVs) from 50 SLE patients and 20 healthy control subjects. The effects of pEVs on NETs formation were evaluated by using immunofluorescence assay and myeloperoxidase-DNA PicoGreen assay. The regulatory mechanism of pEVs on NETs formation and inflammatory cytokines production were investigat

SUBMITTER: Chen YM 

PROVIDER: S-EPMC11229248 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature