Trans-segmental imaging in the spinal cord of behaving mice.
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ABSTRACT:
SUBMITTER: Shekhtmeyster P
PROVIDER: S-EPMC11229418 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Publications
Trans-segmental imaging in the spinal cord of behaving mice.
Shekhtmeyster Pavel P Duarte Daniela D Carey Erin M EM Ngo Alexander A Gao Grace G Olmstead Jack A JA Nelson Nicholas A NA Nimmerjahn Axel A
Nature biotechnology 20230306 12
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm<sup>2</sup> field of view, ~3- to 4-μm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments. ...[more]
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