Carbon-nanotube field-effect transistors for resolving single-molecule aptamer-ligand binding kinetics.

ABSTRACT: Small molecules such as neurotransmitters are critical for biochemical functions in living systems. While conventional ultraviolet-visible spectroscopy and mass spectrometry lack portability and are unsuitable for time-resolved measurements in situ, techniques such as amperometry and traditional field-effect detection require a large ensemble of molecules to reach detectable signal levels. Here we demonstrate the potential of carbon-nanotube-based single-molecule field-effect transistors (smFETs), which can detect the charge on a single molecule, as a new platform for recognizing and assaying small molecules. smFETs are formed by the covalent attachment of a probe molecule, in our case a DNA aptamer, to a carbon nanotube. Conformation changes on binding are manifest as discrete changes in

SUBMITTER: Lee Y 

PROVIDER: S-EPMC11229667 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature