Inhibition of endogenous hydrogen sulfide production reduces activation of hepatic stellate cells via the induction of cellular senescence.

ABSTRACT: In chronic liver injury, quiescent hepatic stellate cells (HSCs) transdifferentiate into activated myofibroblast-like cells and produce large amounts of extracellular matrix components, e.g. collagen type 1. Cellular senescence is characterized by irreversible cell-cycle arrest, arrested cell proliferation and the acquisition of the senescence-associated secretory phenotype (SASP) and reversal of HSCs activation. Previous studies reported that H₂S prevents induction of senescence via its antioxidant activity. We hypothesized that inhibition of endogenous H₂S production induces cellular senescence and reduces activation of HSCs. Rat HSCs were isolated and culture-activated for 7 days. After activation, HSCs treated with H₂S slow-releasing donor GYY4137 and/o

SUBMITTER: Damba T 

PROVIDER: S-EPMC11229775 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature