Multiscale regulation of nutrient stress responses in <i>Escherichia coli</i> from chromatin structure to small regulatory RNAs.

ABSTRACT: Recent research has indicated the presence of heterochromatin-like regions of extended protein occupancy and transcriptional silencing of bacterial genomes. We utilized an integrative approach to track chromatin structure and transcription in E. coli K-12 across a wide range of nutrient conditions. In the process, we identified multiple loci which act similarly to facultative heterochromatin in eukaryotes, normally silenced but permitting expression of genes under specific conditions. We also found a strong enrichment of small regulatory RNAs (sRNAs) among the set of differentially expressed transcripts during nutrient stress. Using a newly developed bioinformatic pipeline, the transcription factors regulating sRNA expression were bioinformatically predicted, with experimental follo

SUBMITTER: Ekdahl AM 

PROVIDER: S-EPMC11230228 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature