Colony context and size-dependent compensation mechanisms give rise to variations in nuclear growth trajectories.

ABSTRACT: To investigate the fundamental question of how cellular variations arise across spatiotemporal scales in a population of identical healthy cells, we focused on nuclear growth in hiPS cell colonies as a model system. We generated a 3D timelapse dataset of thousands of nuclei over multiple days, and developed open-source tools for image and data analysis and an interactive timelapse viewer for exploring quantitative features of nuclear size and shape. We performed a data-driven analysis of nuclear growth variations across timescales. We found that individual nuclear volume growth trajectories arise from short timescale variations attributable to their spatiotemporal context within the colony. We identified a strikingly time-invariant volume compensation relationship between nuclear growth du

SUBMITTER: Dixon JC 

PROVIDER: S-EPMC11230432 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature