Intraoperative spectroscopic evaluation of sentinel lymph nodes in breast cancer surgery.

ABSTRACT:

Background and objectives

Sentinel lymph node (SLN) biopsy is a standard procedure for patients with breast cancer and normal axilla on imaging. Positive SLNs on histological examination can lead to a subsequent surgery for axillary lymph node clearance (ALNC). Here we report a non-destructive technique based on autofluorescence (AF) imaging and Raman spectroscopy for intra-operative assessment of SLNs excised in breast cancer surgery.

Methods

A microscope integrating AF imaging and Raman spectroscopy modules was built to allow scanning of lymph node biopsy samples. During AF-Raman measurements, AF imaging determined optimal sampling locations for Raman spectroscopy measurements. After optimisation of the AF image analysis and training of classification models based on data

SUBMITTER: Barkur S 

PROVIDER: S-EPMC11230987 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature