Prinsepia utilis Royle polysaccharides promote skin barrier repair through the Claudin family.

ABSTRACT:

Background

Plant polysaccharides have various biological activities. However, few studies have been conducted on the skin barrier of Prinsepia utilis Royle polysaccharide extract (PURP).

Materials and methods

The proportions of polysaccharides, monosaccharides and proteins were determined by extracting polysaccharides from fruit meal using water. The healing rate was measured by cell scratch assays. SDS-damaged reconstructed human epidermal models, an acetone-ether-induced mouse model and an IL-4-induced cellular inflammation model were used to detect the effects of polysaccharides on the phenotype, HA, TEWL, and TEER, with further characterizations performed using QRT-PCR, Western blotting, immunofluorescence (IF) assays.

Results

PURP contained 35.73% polysaccharides

SUBMITTER: Wang B 

PROVIDER: S-EPMC11231044 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature