Cellular zinc status alters chromatin accessibility and binding of p53 to DNA.

ABSTRACT: Zn²⁺ is an essential metal required by approximately 850 human transcription factors. How these proteins acquire their essential Zn²⁺ cofactor and whether they are sensitive to changes in the labile Zn²⁺ pool in cells remain open questions. Using ATAC-seq to profile regions of accessible chromatin coupled with transcription factor enrichment analysis, we examined how increases and decreases in the labile zinc pool affect chromatin accessibility and transcription factor enrichment. We found 685 transcription factor motifs were differentially enriched, corresponding to 507 unique transcription factors. The pattern of perturbation and the types of transcription factors were notably different at promoters versus intergenic regions, with zinc-finger transcriptio

SUBMITTER: Ocampo D 

PROVIDER: S-EPMC11231577 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature