Quantitative urinary proteome analysis reveals potential biomarkers for disease activity of Behcet's disease uveitis.

ABSTRACT:

Purpose

Behçet's disease-associated uveitis (BDU) is a severe, recurrent inflammatory condition affecting the eye and is part of a systemic vasculitis with unknown etiology, making biomarker discovery essential for disease management. In this study, we intend to investigate potential urinary biomarkers to monitor the disease activity of BDU.

Methods

Firstly, label-free data-dependent acquisition (DDA) and tandem mass tag (TMT)-labeled quantitative proteomics methods were used to profile the proteomes of urine from active and quiescent BDU patients, respectively. For further exploration, the remaining fifty urine samples were analyzed by a data-independent acquisition (DIA) quantitative proteomics method.

Results

Twenty-nine and 21 differential proteins were identified

SUBMITTER: Qin W 

PROVIDER: S-EPMC11232131 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature