Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α₃DIV-L21C three-helical bundle peptide.

ABSTRACT: The rational design and functionalization of small, simple, and stable peptides scaffolds is an attractive avenue to mimic catalytic metal-centres of complex proteins, relevant for the design of metalloenzymes with environmental, biotechnological and health impacts. The de novo designed α₃DIV-L21C framework has a rubredoxin-like metal binding site and was used in this work to incorporate a Mo-atom. Thermostability studies using differential scanning calorimetry showed an increase of 4 °C in the melting temperature of the Mo-α₃DIV-L21C when compared to the apo-α₃DIV-L21C. Circular dichroism in the visible and far-UV regions corroborated these results showing that Mo incorporation provides stability to the peptide even though there were almost no differences

SUBMITTER: Braganca PMS 

PROVIDER: S-EPMC11232944 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature