A new colorimetric lactate biosensor based on CUPRAC reagent using binary enzyme (lactate-pyruvate oxidases)-immobilized silanized magnetite nanoparticles.

ABSTRACT: A novel optical lactate biosensor is presented that utilizes a colorimetric interaction between H₂O₂ liberated by a binary enzymatic reaction and bis(neocuproine)copper(II) complex ([Cu(Nc)₂]²⁺) known as CUPRAC (cupric reducing antioxidant capacity) reagent. In the first step, lactate oxidase (LOx) and pyruvate oxidase (POx) were separately immobilized on silanized magnetite nanoparticles (SiO₂@Fe₃O₄ NPs), and thus, 2 mol of H₂O₂ was released per 1 mol of the substrate due to a sequential enzymatic reaction of the mixture of LOx-SiO₂@Fe₃O₄ and POx-SiO₂@Fe₃O₄ NPs with lactate and pyruvate, respectively. In the second step, the a

SUBMITTER: Ayaz S 

PROVIDER: S-EPMC11233342 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature