Pharmacokinetic profile of phenytoin in dried blood spot with high-performance liquid chromatography-photodiode array.

ABSTRACT: Phenytoin is a first-line antiepileptic drug with narrow therapeutic range and follows non-linear pharmacokinetics. Pharmacokinetics of phenytoin have been studied in plasma matrix before, however, there were several disadvantages. This study aimed to obtain partial validation data of the analytical method and the pharmacokinetic profile of phenytoin in Dried Blood Spot (DBS) of six healthy subjects. DBS has the advantage of only requiring small sample volumes and could be transported more efficiently. Phenytoin along with carbamazepine as the chosen internal standard was analyzed with a reversed-phase high performance-liquid chromatography system and a photodiode array detector at 205 nm. The results of partial validation, which evaluated the linearity, within-run accuracy, and precision,

SUBMITTER: Harahap Y 

PROVIDER: S-EPMC11233438 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature