UPF1 plays critical roles in early B cell development.

ABSTRACT: The ATP-dependent RNA helicase UPF1 plays a crucial role in various mRNA degradation pathways, most importantly in nonsense-mediated mRNA decay (NMD). Here, we show that UPF1 is upregulated during the early stages of B cell development and is important for early B cell development in the bone marrow. B-cell-specific Upf1 deletion in mice severely impedes the early to late LPre-B cell transition, in which V_H-D_HJ_H recombination occurs at the Igh gene. Furthermore, UPF1 is indispensable for V_H-D_HJ_H recombination, without affecting D_H-J_H recombination. Intriguingly, the genetic pre-arrangement of the Igh gene rescues the differentiation defect in early LPre-B cells under Upf1 deficient conditions. However,

SUBMITTER: Iwai N 

PROVIDER: S-EPMC11233602 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature