Hypertonicity during a rapid rise in D-glucose mediates first-phase insulin secretion.

ABSTRACT:

Introduction

Biphasic insulin secretion is an intrinsic characteristic of the pancreatic islet and has clinical relevance due to the loss of first-phase in patients with Type 2 diabetes. As it has long been shown that first-phase insulin secretion only occurs in response to rapid changes in glucose, we tested the hypothesis that islet response to an increase in glucose is a combination of metabolism plus an osmotic effect where hypertonicity is driving first-phase insulin secretion.

Methods

Experiments were performed using perifusion analysis of rat, mouse, and human islets. Insulin secretion rate (ISR) and other parameters associated with its regulation were measured in response to combinations of D-glucose and membrane-impermeable carbohydrates (L-glucose or mannitol) desi

SUBMITTER: Kamat V 

PROVIDER: S-EPMC11233695 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature