A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed (Brassica napus).

ABSTRACT: Rapeseed is a globally significant oilseed crop cultivated to meet the increasing demand for vegetable oil. In order to enhance yield and sustainability, breeders have adopted the development of rapeseed hybrids as a common strategy. However, current hybrid production systems in rapeseed have various limitations, necessitating the development of a simpler and more efficient approach. In this study, we propose a novel method involving the targeted disruption of Defective in Anther Dehiscence1 of Brassica napus (BnDAD1), an essential gene in the jasmonic acid biosynthesis pathway, using CRISPR/Cas9 technology, to create male-sterile lines. BnDAD1 was found to be dominantly expressed in the stamen of rapeseed flower buds. Disrupting BnDAD1 led to decreased l

SUBMITTER: Tu M 

PROVIDER: S-EPMC11233878 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature