Microglial circ-UBE2K exacerbates depression by regulating parental gene UBE2K via targeting HNRNPU.

ABSTRACT: Background: Knowledge about the pathogenesis of depression and treatments for this disease are lacking. Epigenetics-related circRNAs are likely involved in the mechanism of depression and have great potential as treatment targets, but their mechanism of action is still unclear. Methods: Circular RNA UBE2K (circ-UBE2K) was screened from peripheral blood of patients with major depressive disorder (MDD) and brain of depression model mice through high-throughput sequencing. Microinjection of circ-UBE2K overexpression lentivirus and adeno-associated virus for interfering with microglial circ-UBE2K into the mouse hippocampus was used to observe the role of circ-UBE2K in MDD. Sucrose preference, forced swim, tail suspension and open filed tests were performed to evaluate the depress

SUBMITTER: Cai Y 

PROVIDER: S-EPMC11234284 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature