Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro.

ABSTRACT:

Background

Enamelin is an enamel matrix protein that plays an essential role in the formation of enamel, the most mineralized tissue in the human body. Previous studies using animal models and proteins from natural sources point to a key role of enamelin in promoting mineralization events during enamel formation. However, natural sources of enamelin are scarce and with the current study we therefore aimed to establish a simple microbial production method for recombinant human enamelin to support its use as a mineralization agent.

Results

In the study the 32 kDa fragment of human enamelin was successfully expressed in Escherichia coli and could be obtained using immobilized metal ion affinity chromatography purification (IMAC), dialysis, and lyophilization. This workflow resu

SUBMITTER: Halablab M 

PROVIDER: S-EPMC11234762 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature