Nanoparticle-Enabled Multiplexed Electrochemical Immunoassay for Detection of Surface Proteins on Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs) are lipid bilayer particles secreted from various cells. EVs carry molecular information of parent cells and hold considerable promise for early disease diagnostics. This paper describes a general strategy for multiplexed immunosensing of EV surface proteins, focusing on surface markers CD63, CD81, nephrin, and podocin to prove the concept. This sensing strategy entailed functionalizing gold nanoparticles (AuNPs) with two types of antibodies and then tagging with metal ions, either Pb²⁺ or Cu²⁺. The metal ions served as redox reporters, generating unique redox peaks at -0.23 and 0.28 V (vs Ag/AgCl) during electrochemical oxidation of Pb²⁺ and Cu²⁺, respectively. Capture of EVs on the working electrode, followed by lab

SUBMITTER: Lee S 

PROVIDER: S-EPMC11235089 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature