Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnover.

ABSTRACT: Maps of the RNA modification 5-methylcytosine (m⁵C) often diverge markedly not only because of differences in detection methods, data depand analysis pipelines but also biological factors. We re-analysed bisulfite RNA sequencing datasets from five human cell lines and seven tissues using a coherent m⁵C site calling pipeline. With the resulting union list of 6,393 m⁵C sites, we studied site distribution, enzymology, interaction with RNA-binding proteins and molecular function. We confirmed tRNA:m⁵C methyltransferases NSUN2 and NSUN6 as the main mRNA m⁵C "writers," but further showed that the rRNA:m⁵C methyltransferase NSUN5 can also modify mRNA. Each enzyme recognises mRNA features that strongly resemble their canonical subst

SUBMITTER: Guarnacci M 

PROVIDER: S-EPMC11235314 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature