MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma.

ABSTRACT:

Background

Osteosarcoma is a highly malignant bone tumor that exhibits rapid growth and early metastasis. Hypoxia plays a pivotal role in promoting the proliferation and metastasis of osteosarcoma through a series of molecular events, which are partially mediated and regulated by HIF-1α. However, the regulatory network associated with HIF-1α in osteosarcoma remains limited. Therefore, the objective of this study was to identify critical hypoxia-associated genes and investigate their effects and molecular mechanisms in osteosarcoma cells.

Methods

Through bioinformatics analysis, matrilin-4 (MATN4) was identified as a crucial gene associated with hypoxia. The expression of MATN4 and HIF-1α was assessed using immunohistochemistry, RT-qPCR, and western blotting. The proliferativ

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11236324 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature