HIV Subtypes and Drug-resistance-associated Mutations in US Blood Donors, 2015-2020.

ABSTRACT:

Background

Monitoring genotypes of HIV infections in blood donors may provide insights into infection trends in the general population.

Methods

HIV RNA was extracted from plasma samples of blood donors confirmed as HIV positive by blood screening nucleic acid and antibody tests. HIV genome target regions were amplified using nested real time-polymerase chain reaction followed by next-generation sequencing. Sequences were compared to those in the Los Alamos National Laboratory (LANL) database. Sequences were also assessed for drug resistance mutations (DRM) using the Stanford HIV DRM Database.

Results

From available HIV-positive donations collected between 1 September 2015 and 31 December 2020, 563 of 743 (75.8%) were successfully sequenced; 4 were subtype A, 543 subty

SUBMITTER: Custer B 

PROVIDER: S-EPMC11237352 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature