The importance of choosing the appropriate cholesterol quantification method: enzymatic assay versus gas chromatography.

ABSTRACT: Cholesterol is a major lipid of the animal realm with many biological roles. It is an important component of cellular membranes and a precursor of steroid hormones and bile acids. It is particularly abundant in nervous tissues, and dysregulation of cholesterol metabolism has been associated with neurodegenerative diseases such as Alzheimer's and Huntington's diseases. Deciphering the pathophysiological mechanisms of these disorders often involves animal models such as mice and Drosophila. Accurate quantification of cholesterol levels in the chosen models is a critical point of these studies. In the present work, we compare two common methods, gas chromatography coupled to flame-ionization detection (GC/FID) and a cholesterol oxidase-based fluorometric assay to measure cholesterol in mouse

SUBMITTER: Serrano J 

PROVIDER: S-EPMC11237936 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature