Salvianolic acid B inhibits thrombosis and directly blocks the thrombin catalytic site.

ABSTRACT:

Background

Salvianolic acid B (SAB) is a major component of Salvia miltiorrhiza root (Danshen), widely used in East/Southeast Asia for centuries to treat cardiovascular diseases. Danshen depside salt, 85% of which is made up of SAB, is approved in China to treat chronic angina. Although clinical observations suggest that Danshen extracts inhibited arterial and venous thrombosis, the exact mechanism has not been adequately elucidated.

Objective

To delineate the antithrombotic mechanisms of SAB.

Methods

We applied platelet aggregation and coagulation assays, perfusion chambers, and intravital microscopy models. The inhibition kinetics and binding affinity of SAB to thrombin are measured by thrombin enzymatic assays, intrinsic fluorescence spectrophotometry, and i

SUBMITTER: Neves MAD 

PROVIDER: S-EPMC11238050 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature