Development of a simple and highly sensitive virion concentration method to detect SARS-CoV-2 in saliva.

ABSTRACT:

Background

Controlling novel coronavirus pandemic infection (COVID-19) is a global challenge, and highly sensitive testing is essential for effective control. The saliva is a promising sample for high-sensitivity testing because it is easier to collect than nasopharyngeal swab samples and allows large-volume testing.

Results

We developed a simple SARS-CoV-2 concentration method from saliva samples that can be completed in less than 60 min. We performed a spike test using 12 ml of saliva samples obtained from healthy volunteer people, and the developed method performance was evaluated by comparison using a combination of automatic nucleic acid extraction followed by RT-qPCR detection. In saliva spike tests using a 10-fold dilution series of SARS-CoV-2, the developed method wa

SUBMITTER: Yamazaki Y 

PROVIDER: S-EPMC11238118 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature