Circ_0000069 promotes the development of hepatocellular carcinoma by regulating CCL25.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths globally, influenced by aberrant circRNA expression. Investigating circRNA-miRNA-mRNA interactions can unveil underlying mechanisms of HCC and identify potential therapeutic targets.

Methods

In this study, we conducted differential analyses of mRNAs, miRNAs, and circRNAs, and established their relationships using various databases such as miRanda, miRDB, and miTarBase. Additionally, functional enrichment and immune infiltration analyses were performed to evaluate the roles of key genes. We also conducted qPCR assays and western blotting (WB) to examine the expression levels of circRNA, CCL25, and MAP2K1 in both HCC cells and clinical samples. Furthermore, we utilized overexpression and knoc

SUBMITTER: Zeng J 

PROVIDER: S-EPMC11238365 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature