N⁶-methyladenosine modification of circMARK2 enhances cytoplasmic export and stabilizes LIN28B, contributing to the progression of Wilms tumor.

ABSTRACT:

Background

The potential involvement of circular RNAs (circRNAs) and N⁶-methyladenosine (m⁶A) modification in the progression of Wilms tumor (WT) has not been fully elucidated. This study investigates the regulatory mechanisms and clinical significance of m⁶A-modified circMARK2 and its role in WT progression.

Methods

We identified dysregulated circRNAs through deep sequencing and validated their expression by qRT-PCR in WT tissues. The biological functions of circMARK2 were assessed using clone formation, transwell migration, and orthotopic animal models. To dissect the underlying mechanisms, we employed RNA immunoprecipitation, RNA pull-down, dual-luciferase reporter assays, Western blotting, and immunofluorescence and immunohistochemical st

SUBMITTER: Shu G 

PROVIDER: S-EPMC11238472 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature