Evaluating Chemical Footprinting-Induced Perturbation of Protein Higher Order Structure.

ABSTRACT: Specific amino acid footprinting mass spectrometry (MS) is an increasingly utilized method for elucidating protein higher order structure (HOS). It does this by adding to certain amino acid residues a mass tag, whose reaction extent depends on solvent accessibility and microenvironment of the protein. Unlike reactive free radicals and carbenes, these specific footprinters react slower than protein unfolding. Thus, their footprinting, under certain conditions, provokes structural changes to the protein, leading to labeling on non-native structures. It is critical to establish conditions (i.e., reagent concentrations, time of reaction) to ensure that the structure of the protein following footprinting remains native. Here, we compare the efficacy of five methods in assessing protein HOS foll

SUBMITTER: Wagner WJ 

PROVIDER: S-EPMC11238718 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature