DTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila.

ABSTRACT: The second-generation Drosophila traumatic brain injury (TBI) device dTBI2 improves Drosophila TBI administration by providing a moderate-throughput, tunable, head-specific injury. Our updated device design improves user-friendliness, eliminates inconsistencies in injury timing, and has an updated circuit design to extend the longevity of delicate electronic components. dTBI2 improves reproducibility across users and runs, and results in more consistent post-injury phenotypes. This protocol describes the construction, calibration, and use of the dTBI2 device, which uses an Arduino-controlled piezoelectric actuator to deliver a force that compresses a fly head against a metal collar. The duration and depth of head compression is tunable, allowing calibration of injury severity. All device c

SUBMITTER: Ratner SW 

PROVIDER: S-EPMC11239101 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature