Modulation of cardiac resident macrophages immunometabolism upon high-fat-diet feeding in mice.

ABSTRACT:

Background

A high-fat diet (HFD) contributes to various metabolic disorders and obesity, which are major contributors to cardiovascular disease. As an essential regulator for heart homeostasis, cardiac resident macrophages may go awry and contribute to cardiac pathophysiology upon HFD. Thus, to better understand how HFD induced cardiac dysfunction, this study intends to explore the transcriptional and functional changes in cardiac resident macrophages of HFD mice.

Methods

C57BL/6J female mice that were 6 weeks old were fed with HFD or normal chow diet (NCD) for 16 weeks. After an evaluation of cardiac functions by echocardiography, mouse hearts were harvested and cardiac resident CCR2^- macrophages were sorted, followed by Smart sequencing. Bioinformatics analysis

SUBMITTER: Zhu S 

PROVIDER: S-EPMC11239335 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature