Dynamic establishment and maintenance of the human intestinal B cell population and repertoire following transplantation in a pediatric-dominated cohort.

ABSTRACT:

Introduction

It is unknown how intestinal B cell populations and B cell receptor (BCR) repertoires are established and maintained over time in humans. Following intestinal transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte populations in immunosuppressed conditions.

Methods

Using polychromatic flow cytometry that includes HLA allele group-specific antibodies distinguishing donor from recipient cells along with high throughput BCR sequencing, we tracked the establishment of recipient B cell populations and BCR repertoire in the allograft mucosa of ITx recipients.

Results

We confirm the early presence of naïve donor B cells in the circulation (donor age range: 1-14 years, m

SUBMITTER: Fu J 

PROVIDER: S-EPMC11239347 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature