A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.

ABSTRACT: Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how the activity of such long-range connections contributes to behavior requires efficient methods for reversibly manipulating their function. Chemogenetic and optogenetic tools, acting through endogenous G-protein-coupled receptor pathways, can be used to modulate synaptic transmission, but existing tools are limited in sensitivity, spatiotemporal precision or spectral multiplexing capabilities. Here we systematically evaluated multiple bistable opsins for optogenetic applications and found that the Platynereis dumerilii ciliary opsin (PdCO) is an efficient, versatile, light-activated bistable G-protein-coupled receptor that can suppress synaptic transm

SUBMITTER: Wietek J 

PROVIDER: S-EPMC11239505 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature