Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.

ABSTRACT: The dry mass and the orientation of biomolecules can be imaged without a label by measuring their permittivity tensor (PT), which describes how biomolecules affect the phase and polarization of light. Three-dimensional (3D) imaging of PT has been challenging. We present a label-free computational microscopy technique, PT imaging (PTI), for the 3D measurement of PT. PTI encodes the invisible PT into images using oblique illumination, polarization-sensitive detection and volumetric sampling. PT is decoded from the data with a vectorial imaging model and a multi-channel inverse algorithm, assuming uniaxial symmetry in each voxel. We demonstrate high-resolution imaging of PT of isotropic beads, anisotropic glass targets, mouse brain tissue, infected cells and histology slides. PTI outperforms

SUBMITTER: Yeh LH 

PROVIDER: S-EPMC11239526 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature