A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.

ABSTRACT: Filamentous fungi produce polysaccharide-degrading enzymes, which is controlled by poorly understood transcriptional circuits. Here we show that a circuit comprising RsrC-RsrA-RsrB (Rsr: production of raw-starch-degrading enzyme regulator) that positively regulates production of raw starch-degrading enzymes in Penicillium oxalicum. Transcription factor (TF) RsrA is essential for biosynthesis of raw starch-degrading enzymes. RsrB and RsrC containing Zn2Cys6- and C2H2-zinc finger domains, act downstream and upstream of RsrA, respectively. RsrA activates rsrB transcription, and three nucleotides (G^-286, G^-287 and G^-292) of rsrB promoter region are required for RsrA, in terms of TF, for binding. RsrB_165-271 binds to DNA sequence 5'-TCGATCAGGCACGCC-3'

SUBMITTER: Ning YN 

PROVIDER: S-EPMC11239660 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature