Two newly established and mutually related subfamilies GH13_48 and GH13_49 of the α-amylase family GH13.

ABSTRACT: Currently, the main α-amylase family GH13 has been divided into 47 subfamilies in CAZy, with new subfamilies regularly emerging. The present in silico study was performed to highlight the groups, represented by the maltogenic amylase from Thermotoga neapolitana and the α-amylase from Haloarcula japonica, which are worth of creating their own new GH13 subfamilies. This enlarges functional annotation and thus allows more precise prediction of the function of putative proteins. Interestingly, those two share certain sequence features, e.g. the highly conserved cysteine in the second conserved sequence region (CSR-II) directly preceding the catalytic nucleophile, or the well-preserved GQ character of the end of CSR-VII. On the other hand, the two groups bear also specific and highly conserved

SUBMITTER: Marecek F 

PROVIDER: S-EPMC11239784 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature