Efficient Parahydrogen-Induced ¹³C Hyperpolarization on a Microfluidic Device.

ABSTRACT: We show the direct production and detection of ¹³C-hyperpolarized fumarate by parahydrogen-induced polarization (PHIP) in a microfluidic lab-on-a-chip (LoC) device and achieve 8.5% ¹³C polarization. This is the first demonstration of ¹³C-hyperpolarization of a metabolite by PHIP in a microfluidic device. LoC technology allows the culture of mammalian cells in a highly controlled environment, providing an important tool for the life sciences. In-situ preparation of hyperpolarized metabolites greatly enhances the ability to quantify metabolic processes in such systems by microfluidic NMR. PHIP of ¹H nuclei has been successfully implemented in microfluidic systems, with mass sensitivities in the range of pmol/s. However, metabolic NMR requires high-

SUBMITTER: Barker SJ 

PROVIDER: S-EPMC11240250 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature