Isolation, Characterization and IgE Binding of Two 2S Albumins of Pomegranate Seeds.

ABSTRACT: Literature reports suggest that the presence of proteins in pomegranate seeds is responsible for sensitization and IgE-mediated allergic reactions. The objective of this study was the analysis of a pomegranate seed extract and the isolation and characterization of proteins contained in high amounts. The extract characterization showed a protein profile with main bands at about 18 kDa and below 10 kDa upon SDS-PAGE, and molecules were recognized by specific IgEs upon immunoblotting. Then, two new 2S albumins, a monomeric and a heterodimeric one, were isolated by using classical biochemical methods. They were identified via direct protein sequencing and mass spectrometry, and their primary structure was analyzed and compared with homologous allergenic proteins via bioinformatics. In an Itali

SUBMITTER: Tuppo L 

PROVIDER: S-EPMC11241328 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature