Within-laboratory reproducibility of Ames test results: Are repeat tests necessary?

ABSTRACT: The Ames test is required by regulatory agencies worldwide for assessing the mutagenic and carcinogenic potential of chemical compounds. This test uses several strains of bacteria to evaluate mutation induction: positive results in the assay are predictive of rodent carcinogenicity. As an initial step to understanding how well the assay may detect mutagens present as constituents of complex mixtures such as botanical extracts, a cross-sector working group examined the within-laboratory reproducibility of the Ames test using the extensive, publicly available National Toxicology Program (NTP) Ames test database comprising more than 3000 distinct test articles, most of which are individual chemicals. This study focused primarily on NTP tests conducted using the standard Organization for Econo

SUBMITTER: Zeiger E 

PROVIDER: S-EPMC11241522 | biostudies-literature | 2024 Mar-Apr

REPOSITORIES: biostudies-literature