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Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024.

ABSTRACT: We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.

SUBMITTER: Beck AS

PROVIDER: S-EPMC11241853 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024.

Beck Andrew S AS Lopareva Elena N EN Hwang Hyun H Hart Derek D de Almeida Marcos M Anderson Raydel R Rota Paul A PA Bankamp Bettina B

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 20240701 28

We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensi ...[more]

PMID: 38994600

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