Project description:Robotic positioning systems are used in a variety of chemical instruments, primarily for liquid handling purposes, such as autosamplers from vials or well plates. Here, two approaches to the design of open-source autosampler positioning systems for use with 96-well plates are described and compared. The first system, a 3-axis design similar to many low-cost 3D printers that are available on the market, is constructed using an aluminum design and stepper motors. The other system relies upon a series of 3D printed parts to achieve movement with a series of linker arms based on Selective Compliance Assembly Robot Arm (SCARA) design principles. Full printer design files, assembly instructions, software, and user directions are included for both samplers. The positioning precision of the 3-axis system is better than the SCARA mechanism due to finer motor control, albeit with a slightly higher cost of materials. Based on the improved precision of this approach, the 3-axis autosampler system was used to demonstrate the generation of a segmented flow droplet stream from adjacent wells within a 96-well plate.
Project description:Rheology describes the flow of fluids from food and plastics, to coatings, adhesives, and 3D printing inks, and is commonly denoted by viscosity alone as a simplification. While viscometers adequately probe Newtonian (constant) viscosity, most fluids have complex viscosity, requiring tests over multiple shear rates, and transient measurements. As a result, rheometers are typically large, expensive, and require additional infrastructure (e.g., gas lines), rendering them inaccessible for regular use by many individuals, small organizations, and educators. Here, we introduce a low-cost (under USD$200 bill of materials) Open Source Rheometer (OSR), constructed entirely from thermoplastic 3D printed components and off-the-shelf electromechanical components. A sample fluid rests in a cup while a micro stepping motor rotates a tool inside the cup, applying strain-controlled shear flow. A loadcell measures reaction torque exerted on the cup, and viscosity is calculated. To establish the measurement range, the viscosity of four Newtonian samples of 0.1-10 Pa.s were measured with the OSR and compared to benchmark values from a laboratory rheometer, showing under 23% error. Building on this, flow curves of three complex fluids - a microgel (hand sanitizer), foam (Gillette), and biopolymer solution (1% Xanthan Gum) - were measured with a similar error range. Stress relaxation, a transient test, was demonstrated on the biopolymer solution to extract the nonlinear damping function. We finally include detailed exposition of measurement windows, sources of error, and future design suggestions. The OSR cost is ∼1/25th that of commercially available devices with comparable minimum torque (200 µN.m), and provides a fully open-source platform for further innovation in customized rheometry.
Project description:Fluorescent imaging with voltage- or calcium-sensitive dyes, known as optical mapping, is one of the indispensable modern techniques to study cardiac or neural electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200x200 977 frames per second (FPS) action potential recordings from rodent hearts, with the signal-to-noise-ratio of a conditioned signal of 16 ± 10. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) while lower is sufficient for accurate measurements of AP waveform, conduction velocity (± 0.04 m/s) and action potential duration (± 7ms) in mouse and rat hearts. We used 4-aminopyridine to prolong the action potential duration in mouse heart, thus demonstrating that the proposed system is adequate for pharmacological studies.
Project description:Microencapsulation is a widely studied cell therapy and tissue bioengineering technique, since it is capable of creating an immune-privileged site, protecting encapsulated cells from the host immune system. Several polymers have been tested, but sodium alginate is in widespread use for cell encapsulation applications, due to its low toxicity and easy manipulation. Different cell encapsulation methods have been described in the literature using pressure differences or electrostatic changes with high cost commercial devices (about 30,000 US dollars). Herein, a low-cost device (about 100 US dollars) that can be created by commercial syringes or 3D printer devices has been developed. The capsules, whose diameter is around 500 µm and can decrease or increase according to the pressure applied to the system, is able to maintain cells viable and functional. The hydrogel porosity of the capsule indicates that the immune system is not capable of destroying host cells, demonstrating that new studies can be developed for cell therapy at low cost with microencapsulation production. This device may aid pre-clinical and clinical projects in low- and middle-income countries and is lined up with open source equipment devices.
Project description:Spectroradiometry is a vital tool in a wide range of biological, physical, astronomical and medical fields, yet its cost and accessibility are frequent barriers to use. Research into the effects of artificial light at night (ALAN) further compounds these difficulties with requirements for sensitivity to extremely low light levels across the ultraviolet to human-visible spectrum. Here, I present an open-source spectroradiometry (OSpRad) system that meets these design challenges. The system utilises an affordable miniature spectrometer chip (Hamamatsu C12880MA), combined with an automated shutter and cosine-corrector, microprocessor controller, and graphical user interface 'app' that can be used with smartphones or desktop computers. The system has high ultraviolet sensitivity and can measure spectral radiance at 0.001 cd m-2 and irradiance at 0.005 lx, covering the vast majority of real-world night-time light levels. The OSpRad system's low cost and high sensitivity make it well suited to a range of spectrometry and ALAN research.
Project description:Microbial electrochemical technologies (MET) are emerging systems for environmental applications such as renewable energy production or pollution remediation. MET research often requires stable temperatures and low levels of electromagnetic interference. Due to the presence of electrical wires and sensors, heating MET using water jacket recirculation can raise safety issues, whereas heating coils may affect the results of electrochemical analyses. The proposed open-source temperature-control chamber (OpenTCC) aims to provide a low-cost solution for controlling temperature (in the range 20-55 °C) while simultaneously reducing the electromagnetic interferences caused by switching mode power supplies. OpenTCC consists of a light and cheap structure, incorporating eight heating pads and two Peltier-cooling modules powered by open-source electronic circuits. Its hardware is controlled by an Arduino microcontroller and a Python interface which provides data-logging and serve as a basis for programable temperature cycles. The system has a modular design to allow stacking several independent modules. OpenTCC provides a reliable and tunable temperature control at lower costs than currently available commercial temperature controllers and provides a platform for field-specific upgrades. Though optimized for MET, Open-TCC can be adapted to other laboratory applications due to its flexible design.
Project description:The increasing transformation of biodiversity into a data-intensive science has seen numerous independent systems linked and aggregated into the current landscape of biodiversity informatics. This paper outlines how we can move forward with this programme, incorporating real time environmental monitoring into our methodology using low-power and low-cost computing platforms.
Project description:Spectrophotometry is a fundamental technique in many areas of science, with many applications and uses. The cost of spectrophotometers has acted as a barrier on the teaching and use of the technique. Here, we provide open-source plans to a 3D-printed cuvette holder with an interchangeable narrow-spectral bandwidth light-emitting diode (LED) block that can be used in conjunction with a smartphone's ambient light sensor (ALS) to perform spectrophotometry. A Lego version with an interchangeable LED block is also presented. Results from the smartphone spectrophotometer in comparison with commercially available spectrophotometers demonstrated functionality, and the model may have many applications, especially in indirect spectrophotometry, such as in the protein assay shown here. The plans for the 3D-printed model are freely available on GitHub, as are editable files to allow customisation by users. We would encourage users to share adaptations with the scientific community.
Project description:BackgroundRemote monitoring of plants using hyperspectral imaging has become an important tool for the study of plant growth, development, and physiology. Many applications are oriented towards use in field environments to enable non-destructive analysis of crop responses due to factors such as drought, nutrient deficiency, and disease, e.g., using tram, drone, or airplane mounted instruments. The field setting introduces a wide range of uncontrolled environmental variables that make validation and interpretation of spectral responses challenging, and as such lab- and greenhouse-deployed systems for plant studies and phenotyping are of increasing interest. In this study, we have designed and developed an open-source, hyperspectral reflectance-based imaging system for lab-based plant experiments: the HyperScanner. The reliability and accuracy of HyperScanner were validated using drought and salt stress experiments with Arabidopsis thaliana.ResultsA robust, scalable, and reliable system was created. The system was built using open-sourced parts, and all custom parts, operational methods, and data have been made publicly available in order to maintain the open-source aim of HyperScanner. The gathered reflectance images showed changes in narrowband red and infrared reflectance spectra for each of the stress tests that was evident prior to other visual physiological responses and exhibited congruence with measurements using full-range contact spectrometers.ConclusionsHyperScanner offers the potential for reliable and inexpensive laboratory hyperspectral imaging systems. HyperScanner was able to quickly collect accurate reflectance curves on a variety of plant stress experiments. The resulting images showed spectral differences in plants shortly after application of a treatment but before visual manifestation. HyperScanner increases the capacity for spectroscopic and imaging-based analytical tools by providing more access to hyperspectral analyses in the laboratory setting.
Project description:We describe the "FishCam", a low-cost ( < 500 USD) autonomous camera package to record videos and images underwater. The system is composed of easily accessible components and can be programmed to turn ON and OFF on customizable schedules. Its 8-megapixel camera module is capable of taking 3280 × 2464-pixel images and videos. An optional buzzer circuit inside the pressure housing allows synchronization of the video data from the FishCam with passive acoustic recorders. Ten FishCam deployments were performed along the east coast of Vancouver Island, British Columbia, Canada, from January to December 2019. Field tests demonstrate that the proposed system can record up to 212 h of video data over a period of at least 14 days. The FishCam data collected allowed us to identify fish species and observe species interactions and behaviors. The FishCam is an operational, easily-reproduced and inexpensive camera system that can help expand both the temporal and spatial coverage of underwater observations in ecological research. With its low cost and simple design, it has the potential to be integrated into educational and citizen science projects, and to facilitate learning the basics of electronics and programming.