Cell type- and transcription-independent spatial proximity between enhancers and promoters.

ABSTRACT: Cell type-specific enhancers are critically important for lineage specification. The mechanisms that determine cell-type specificity of enhancer activity, however, are not fully understood. Most current models for how enhancers function invoke physical proximity between enhancer elements and their target genes. Here, we use an imaging-based approach to examine the spatial relationship of cell type-specific enhancers and their target genes with single-cell resolution. Using high-throughput microscopy, we measure the spatial distance from target promoters to their cell type-specific active and inactive enhancers in individual pancreatic cells derived from distinct lineages. We find increased proximity of all promoter-enhancer pairs relative to non-enhancer pairs separated by similar genomic

SUBMITTER: Mian Y 

PROVIDER: S-EPMC11244156 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature