Nkd1 functions downstream of Axin2 to attenuate Wnt signaling.

ABSTRACT: Wnt signaling is a crucial developmental pathway involved in early development as well as stem-cell maintenance in adults and its misregulation leads to numerous diseases. Thus, understanding the regulation of this pathway becomes vitally important. Axin2 and Nkd1 are widely utilized negative feedback regulators in Wnt signaling where Axin2 functions to destabilize cytoplasmic β-catenin, and Nkd1 functions to inhibit the nuclear localization of β-catenin. Here, we set out to further understand how Axin2 and Nkd1 regulate Wnt signaling by creating axin2^gh1/gh1, nkd1^gh2/gh2 single mutants and axin2^gh1/gh1;nkd1^gh2/gh2 double mutant zebrafish using sgRNA/Cas9. All three Wnt regulator mutants were viable and had impaired heart loo

SUBMITTER: Bell I 

PROVIDER: S-EPMC11244159 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature