A Highly Efficient <i>Agrobacterium rhizogenes</i>-Mediated Hairy Root Transformation Method of <i>Idesia polycarpa</i> and the Generation of Transgenic Plants.

ABSTRACT: Idesia polycarpa is a promising woody oilseed species because of its high oil yield. However, its use is greatly limited due to the lack of varieties with good qualities; additionally, gene function has been less studied in this plant because an efficient transformation method has not been established yet. In this study, we established a rapid and efficient hairy root transformation method by infecting the whole seedling, the rootless seedling, and the leaf petiole with Agrobacterium rhizogenes using different infection methods. Among these transformation methods, a higher transformation efficiency was obtained using the whole seedling, which could reach up to 71.91%. Furthermore, we found that the seedling age significantly affected the transformation efficiency, either usin

SUBMITTER: Wang H 

PROVIDER: S-EPMC11244318 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature